Red-Headed Mouse Spiders are very defensive and short-tempered animals. When they feel threatened, they will rear-up in a defensive posture and show the bright colouration of their chelicerae and bare their fangs as a warning. However they are by no means aggressive animals, and should not be labelled as such. 

Due to their rarity, and tendency to inflict 'dry bites' (bites with no venom injected) as a warning, there have been very few cases of actual envenomation occurring as a result of bites from these spiders, however, their venom is considered medically significant and is theorised that it could even be as potent as that of its cousin the 'Sydney Funnel Web Spider' (A. robustus). Because of this, should you be bitten medical attention should be sought immediately. Luckily, no deaths have ever occurred from a Red-Headed Mouse Spider bite and they are easily treated with antivenin. 

Should a Red-Headed Mouse Spider be encountered it is advised against killing it, as males are commonly seen when they emerge from their burrows to breed. They can be collected from your home and easily released outside with the plastic (not paper due to their large fangs) under cup method. Otherwise, it is advised to observe from a safe distance so as to not disturb the spider.

females of this species are considered to be more toxic than males.

Male Red-Headed Mouse Spiders commonly emerge from their burrows during late summer to early autumn to breed in which they will wander large areas with their pedipalps postured forwards to detect pheromone trails to locate females. They are unusual from other Mygalomorphs as their usually roam during the day whereas other spiders of this classification are nocturnal in this behaviour. 

Once the burrow of a female has been found, males will tap in a unique rhythmic pattern on the silk lining on the end of the burrow known as 'drumming'. This unique pattern helps to inform the females that the males is here to mate, and not food. If the females is receptive, the male spider follows the female back into the burrow where mating occurs by the male inserting its pedipalps into specialised organs on the underside of the female. This is a risky manoeuvre, as it puts the male at the mercy of the females fangs. Specialised spurs on the end of the male's front legs assist in holding the female in place while they breed. Sometimes this is unsuccessful, and they female will proceed to overpower and eat the male. Luckily, the male oftentimes has succeeded in its task before this occurs.

Females will lay up to 60 eggs in an egg-sac woven from silk, which is places in the brood chamber which branches off the main shaft of her burrow.  The spiderlings (slings) hatch over the remainder of the breeding months and remain with their mother until winter after which they will disperse independently through a technique known as 'ballooning'.  

If you are bitten, the bite should be treated as one that is as potent as that of a Funnel-Web Spider until more information on their toxicity is available. The circulation of venom through the system can be slowed with the swift application of bandages wrapped tightly around the site of the bite, moving upwards as high as possible. The victim should avoid moving as much as possible and medical attention should be sought immediately.

Do not wash residue venom off the skin, as it can assist in the identification of the spider which can result in swifter and more effective treatment. Because of this, collection of the specimen is recommended if possible.